CHFR monoclonal antibody (M01), clone 1H3-A12

SKU: orb2291009

Description

Mouse monoclonal antibody raised against a full length recombinant CHFR.

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 1H3-A12
• Immunogen: CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

CHFR monoclonal antibody (M01), clone 1H3-A12. Western Blot analysis of CHFR expression in 293.

Detection limit for recombinant GST tagged CHFR is approximately 0.1 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12. Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # orb2291009). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (97.46 KDa).

Quick Database Links

RefSeq:AAH12072.1