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Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
SKU: orb346895
Description
Research Area
Product Categories/Products/Antibodies,Immunogen Type: Native Protein
Images & Validation
−| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Gallus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Alk Phos Chicken Antibody, Gt-a-Chicken IgG Alkaline Phosphatase, Chicken Antibody in Goat, Chicken Secondary Antibody, Alk Phos Gt anti-Ch IgY Antibody, Goat Anti-Chicken IgY Alkaline Phosphatase,
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−Chicken IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed [orb346929]
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot


