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Chromogranin A Antibody [CHGA/765]

SKU: orb1252584

Description

Chromogranin A Antibody [CHGA/765]

Research Area

,Cancer,Neuroscience,Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC-P, WB
ReactivityHuman
Application Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Chromogranin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone No.CHGA/765
ImmunogenRecombinant human protein was used as the immunogen for the Chromogranin A antibody.
TargetCHGA
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, ER-37, CHGA

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Chromogranin A Antibody [CHGA/765]

Formalin-fixed, paraffin-embedded human pancreas stained with Chromogranin A antibody (CHGA/765).

Chromogranin A Antibody [CHGA/765]

Formalin-fixed, paraffin-embedded human parathyroid stained with Chromogranin A antibody (CHGA/765).

Chromogranin A Antibody [CHGA/765]

Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A antibody (CHGA/765).

Chromogranin A Antibody [CHGA/765]

SDS-PAGE Analysis of Purified, BSA-Free Chromogranin A Antibody (clone CHGA/765). Confirmation of Integrity and Purity of the Antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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FC
Flow Cytometry
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IF
Immunofluorescence
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Chromogranin A Antibody [CHGA/765] (orb1252584)

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Available Sizes

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100 μg
$ 1,070.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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