Cleaved LC3B Antibody

SKU: orb33337

Description

Rabbit polyclonal antibody to Cleaved LC3

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Neuroscience,Signaling Pathways,Signaling Pathways/Autophagic,Epigenetics,Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Metabolism;Neuroscience;Signal Transduction;Autophagy;Autophagy

Images & Validation

• Tested Applications: ICC, IF
• Dilution Range: IF/ICC: 1:10-50, IF/ICC-1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15839;RB28608
• Immunogen: Synthetic Peptide
• Target: MAP1LC3B
• Molecular Weight: 14688
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATG8F antibody, anti Autophagy-related protein LC3 B antibody, anti Autophagy-related ubiquitin-like modifier LC3 B antibody, anti LC3 antibody, anti LC 3 antibody, anti LC3A antibody, anti LC3B antibody, anti LC3II antibody, anti MAP1 light chain 3 like protein 2 antibody, anti MAP1 light chain 3-like protein 2 antibody, anti MAP1A/1BLC3 antibody, anti MAP1A/MAP1B LC3 B antibody, anti MAP1A/MAP1B light chain 3 B antibody, anti MAP1ALC3 antibody, anti MAP1LC3B a antibody, anti Map1lc3b antibody, anti Microtubule associated protein 1 light chain 3 beta antibody, anti Microtubule-associated protein 1 light chain 3 beta antibody, anti Microtubule-associated proteins 1A/1B light chain 3B antibody

SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100.

Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures.

Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Quick Database Links

Gene Symbol

MAP1LC3B

UniProt

Q9GZQ8

RefSeq (Protein):NP_073729.1