CMA1/Mast Cell Chymase Antibody

SKU: orb1538251

Description

CMA1/Mast Cell Chymase Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: IHC-P (2.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1 µg/ml)
• Reactivity: Human, Monkey
• Application Notes:
  Further information: Immunohistochemistry: LS-B4134 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4134 was determined to be 1.5 to 3.75 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the protein sequence according to NP_001827.1.
• Target: CMA1 / Mast Cell Chymase
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Quick Database Links

Gene Symbol

CMA1 / Mast Cell Chymase