COL1A1 / Collagen I

SKU: orb1294293

Description

Rabbit polyclonal antibody to COL1A1

Research Area

,Product Categories/Antibodies,ECM Profiling / EMT

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000-1:1500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100–1:300
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: COL1A1 / Collagen I
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

COL1A1/Collagen I antibody - at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1/Collagen I antibody (red) at 1:400 and 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1/Collagen I with orb1294293 at 1/150.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1/Collagen I with orb1294293 at 1/150.

Anti-COL1A1/Collagen I antibody - at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1/Collagen I with orb1294293 at 1/100.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1/Collagen I with orb1294293 at 1/100.

COL1A1/Collagen I antibody - at 1/250 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Quick Database Links

Gene Symbol

COL1A1 / Collagen I