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COL2A1 antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB:1:200, IF/ICC: 1:300, IHC-P:1:300 (based on 0.5 mg/ml) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human COL2A1. Please contact us for the exact immunogen sequence. The peptide is available as orb374716. |
| Target | COL2A1 |
| Molecular Weight | 117 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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IHC-P staining of pig liver tissue using Collagen II antibody (dilution at 1:300)

IF analysis of rabbit meniscus tissue using Collagen II antibody (dilution of primary antibody at 1:300)

Immunohistochemical analysis of formalin fixed and paraffin embedded rat intervertebral disc using Collagen II antibody (primary antibody at 1:300)

Immunohistochemical analysis of paraffin-embedded collagen of rat knee joint using Collagen II antibody (dilution of primary antibody - 1:300)

IHC-P of rat knee joint collagen (Collagen II antibody at 1:200)

Western blot analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (primary antibody at 1:200)

IHC-P of human esophageal carcinoma (Collagen II antibody atb 1:300)

Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue using Collagen II antibody (primary antibody at 1:300)

WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2) using Collagen II antibody (dilution of primary antibody - 1:100)

Immunofluorescence analysis of mouse brain tissue using anti-Collagen II (dilution of primary antibody - 1:300)

IF image of mouse lung tissue using anti-Collagen II (primary antibody at 1:300)

IF analysis of mouse lung tissue using Collagen II antibody (dilution of primary antibody at 1:300)

Immunohistochemical staining of mouse lung tissue using anti-Collagen II (dilution of primary antibody - 1:300)

Immunofluorescence image of mouse brain tissue using anti-Collagen II (dilution at 1:300)
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Protocol Information
Kuo, Chang-Yi et al. Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering Carbohydr Polym, 117, 722-730 (2015)
COL2A1 antibody (orb10436)
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