COL4A1 Antibody

SKU: orb1263273

Description

COL4A1 Antibody

Research Area

,Cancer,Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IF starting dilution is: 1:25

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1.
• Target: COL4A1
• Molecular Weight: 161 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha-1(IV) chain, Arresten, COL4A1

Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane.

Fluorescent image of U251 cells stained with COL4A1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using COL4A1 Antibody at 1:1000 at each lane.

Quick Database Links

Gene Symbol

COL4A1

UniProt

P02462

RefSeq:P02462