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Description
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Gallus, Porcine, Rabbit, Sheep |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human COL5A1 (1401-1500/1838aa) |
| Target | COL5A1 |
| Molecular Weight | 153 kDa |
| Purification | affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Skin tissue lysates, Lane 3: Mouse Uterus tissue lysates, Lane 4: Rat Uterus tissue lysates, Lane 5: Human A549 cell lysates, Primary: Anti-COL5A1 (orb10443) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kDa, Observed band size: 200 kDa.
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UniProt Details
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Protocol Information
COL5A1 Rabbit Polyclonal Antibody (orb10443)
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