Collagen II Antibody / COL2A1

SKU: orb1825750

Description

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: COL2A1/8882R
• Immunogen: Recombinant full-length human COL2A1 protein was used as the immunogen for the COL2A1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the COL2A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This COL2A1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human kidney tissue with COL2A1 antibody (clone COL2A1/8882R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P02458