Collagen II Rabbit Polyclonal Antibody

SKU: orb156419

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human
Predicted Reactivity	Gallus, Mouse, Rabbit

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from rabbit Collagen II Chondrocalcin (1401-1487/1487aa)
Target	COL2A1
Molecular Weight	117/163 kDa
Purification	affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Chondrocalcin; Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC.

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Sample: HepG2 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb156419) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.

Sample: U2OS Cell (Human) Lysate at 40 ug, MDA-MB-231 Cell (Human) Lysate at 40 ug, Primary: Anti-Collagen II (orb156419) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117/163 kD, Observed band size: 100 kD.

Quick Database Links

Gene Symbol

COL2A1

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Protocol Information

Western Blot (IB, immunoblot)

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