Collagen IV Antibody / COL4A1/2

SKU: orb1151657

This product can be custom made

Description

Mouse monoclonal antibody to Collagen IV

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Epigenetics

Images & Validation

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Tested Applications	IF, IHC-P, WB
Dilution Range	Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the Collagen IV antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	M3F7
Immunogen	An amino acid sequence from the alpha1 (IV)-alpha2(IV) triple helix was used as the immunogen for the Collagen IV antibody. This antibody does not recognize denatured type IV collagen.
Purity	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Aliquot the Collagen IV antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Hazard Information	This Collagen IV antibody is available for research use only.
Disclaimer	For research use only

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IHC staining of FFPE human kidney tissue with Collagen IV antibody (clone M3F7). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney adenocarcinoma tissue with Collagen IV antibody (clone M3F7) at 2 ug/ml in PBS for 30 min RT. Strong staining of glomeruli is observed. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.