Collagen IV Antibody / COL4A5

SKU: orb2636057

Description

Type IV collagen is the major structural component of the glomerular basement membrane (GBM), forming a hexagonal network together with lamins, proteoglycans, and nestin. Deletions of the N-terminal regions of COL4A5 and COL4A6 were found in Xq22.3 chromosome deletion syndrome and were located in an end-to-end manner. This results in a phenotype characteristic of diffuse leiomyomatosis and Alport syndrome (DL-ATS).

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the COL4A5 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: COL4A5/9396R
• Immunogen: A recombinant fragment (within amino acids 1400-1685) of human COL4A protein was used as the immunogen for the COL4A5 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This COL4A5 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human pancreas tissue with COL4A5 antibody (clone COL4A5/9396R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free COL4A5 (COL4A5/9396R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P29400