Collagen IV antibody (MSVA-704R)

SKU: orb2276391

Description

Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen. Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells. Cellular Localization: Cytoplasmic and extracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-704R
• Immunogen: Purified human placental extract
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RATOR

Collagen IV positive membranes surround pancreatic acini. Small blood vessels do also stain positive.

In the intestine collagen IV immunostaining involves basement membranes vessels of all sizes and smooth muscle cells of the lamina muscularis mucosae.

Papillary renal cell carcinoma showing a delicate collagen IV positive membrane between tumor cells and the stroma and an intense staining of vessels.

Quick Database Links

UniProt

P02462, P53420, P29400, P08572