Collagen IV Antibody

SKU: orb1537863

Description

Collagen IV Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, IP, WB
• Dilution Range: ELISA (1:5000 - 1:50000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:1000 - 1:10000)
• Reactivity: Bovine, Human, Mammal
• Application Notes:
  Further information: Immunohistochemistry: LS-B2717 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2717 was determined to be 2.5 ug/ml.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Collagen Type IV from human and bovine placenta.
• Target: Collagen IV
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Collagen IV