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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000 - 1:1500 Immunofluorescence 1:300 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:200 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide |
| Target | Collagen IV /COL4A1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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COL4A1/Collagen IV antibody - at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1735106 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling COL4A1/Collagen IV with orb1735106 at 1/100.

Immunohistochemical analysis of paraffin embedded Human placenta labeling COL4A1/Collagen IV with orb1735106 at 1/100.

Immunohistochemical analysis of paraffin embedded Human placenta labeling COL4A1/Collagen IV with orb1735106 at 1/100.
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Protocol Information
Collagen IV /COL4A1 (orb1735106)
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