Collagen Type II Antibody Peroxidase Conjugated

SKU: orb345845

Description

CO1A2 antibody

Research Area

Product Categories/Products/Antibodies,Immunogen Type: Native Protein

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:5,000 - 1:10,000
• Reactivity: Bovine, Human
• Application Notes:
  Anti-Collagen Type II Peroxidase Conjugated Antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Collagen Type II antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody

Quick Database Links

UniProt

P02458

RefSeq (Protein):NP_001835.3