Collagen Type VI, human

SKU: orb867273

Description

Rabbit polyclonal antibody to Collagen Type VI

Research Area

Cancer Research

Images & Validation

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Tested Applications	ELISA, IF, IHC-P, RIA, WB
Dilution Range	(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)
Application Notes	Positive Control: Human placenta. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Immunogen	Purified collagen type VI from human placenta
Purification	Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Affinity purified antibody lyophilized from phosphate buffered solution; no rAlbumin and preservative added!
Concentration	app. 1 mg/ml
Expiration Date	12 months from date of receipt.
Hazard Information	*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Disclaimer	For research use only

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Human Collagen Type VI protein [orb750386]

Applications:	ELISA, IHC, SDS-PAGE, WB
Conjugation:	Unconjugated
Purity:	Human Collagen type VI has been prepared from Human Placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type VI. Reaction with Rockland’s anti-Collagen I, II, III, IV or V is negligible (typically less than 1% cross reactivity was detected by ELISA).
Source:	Human

Sizes Available: 500 μg

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Human Collagen Type VI Alpha 1 (COL6a1) ELISA Kit [orb776947]
Reactivity:
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Dynamic Range:
62.5-4000 pg/mL
Sensitivity:
24.6 pg/mL
Sizes Available: 96 T, 48 T
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Human Collagen Type VI Alpha 3 (COL6a3) ELISA Kit [orb777477]
Reactivity:
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Dynamic Range:
31.25-2000 pg/mL
Sensitivity:
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Sizes Available: 96 T, 48 T
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Human Collagen Type VI (COL6) ELISA Kit [orb776841]
Reactivity:
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Dynamic Range:
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Human Collagen Type Ⅵ Alpha 3 (COL6α3) ELISA Kit [orb1949409]
Reactivity:
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