Collagen VII Antibody

SKU: orb2310030

Description

Collagen VII Antibody

Research Area

,Cardiovascular

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA (For coating, order antibody without BSA); Western Blot (0.5-1.0ug/ml);,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Bovine, Equine, Goat, Guinea pig, Human, Sheep
• Application Notes:
  Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: LH7.2
• Immunogen: Recombinant full-length human Collagen VII protein
• Target: COL7A1
• Source/Expression System: CHO cells
• Molecular Weight: 290kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen

Quick Database Links

Gene Symbol

COL7A1

UniProt

Q02388

NCBI Gene ID

1294