COXIV Antibody

SKU: orb1925391

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Cancer;Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:25, WB: 1:2000
• Reactivity: Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB58106
• Target: This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the mouse region of mouse COXIV.
• Molecular Weight: 19530 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a

Anti-COXIV Antibody at 1:2000 dilution + BA/F3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Cox4i1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the mouse region of mouse COXIV.

UniProt

P19783