CREB1 monoclonal antibody (M02), clone 5F2

SKU: orb2294175

Description

Mouse monoclonal antibody raised against a partial recombinant CREB1.

Images & Validation

• Tested Applications: ELISA, IHC-P, PLA, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 5F2
• Immunogen: CREB1 (NP_004370, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQISTIAESE
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged CREB1 is approximately 1 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to CREB1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 ug/ml].

Proximity Ligation Analysis of protein-protein interactions between TP53 and CREB1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CREB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of CREB1 expression in transfected 293T cell line by CREB1 monoclonal antibody (M02), clone 5F2. Lane 1: CREB1 transfected lysate(37 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.19 KDa).

Quick Database Links

RefSeq (Protein):NP_004370