CREB5 Antibody / CRE-BPa

SKU: orb2636875

Description

The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the CREB5 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: PCRP-CREB5-1G8
• Immunogen: Recombinant full-length human CREB5 protein was used as the immunogen for the CREB5 antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CREB5 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q02930