Cyclin D1

SKU: orb1294339

Description

Rabbit polyclonal antibody to Cyclin D1

Research Area

,Product Categories/Antibodies,Cell cycle

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:100–1:200
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Cyclin D1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-Cyclin D1 antibody at 1/1000 dilution, Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294339 overnight at 4°C.

Anti-Cyclin D1 antibody at 1/1000 dilution, Lysates at 30 μg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294339 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with orb1294339 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cyclin D1 with orb1294339 at 1/200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294339 Anti-Cyclin D1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Cyclin D1