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Description
Research Area
,Recombinant-Protein
Images & Validation
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Key Properties
−| Expression System | E. coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase). CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. |
| Tag | C-6xHis |
| Expression Region | Gly2-Glu105 |
| MW | 16 KDa (reducing condition) |
| Purity | 98.00% |
| Protein Sequence | Gly2-Glu105 |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant human Cytochrome C protein, His & MBP [orb1516790]
> 95% as determined by SDS-PAGE
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100 μg, 500 μg Recombinant Human CYCS Protein, His Tag [orb1552569]
≥90% as determined by SDS-PAGE
This protein contains the human CYCS(Gly2-Glu105) was fused with the C-terminal His Tag and expressed in E. coli.
100 μg, 50 μg
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Protocol Information
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