Cytochrome C Antibody

SKU: orb639865

Description

In mammalian cells, the highly conserved cytochrome C protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml,Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: CYCS/3128R
• Immunogen: A recombinant full-length human protein was used as the immunogen for this recombinant Cytochrome C antibody.
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the recombinant Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant Cytochrome C antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant Cytochrome C antibody (clone CYCS/3128R, green).

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytochrome C antibody (clone CYCS/3128R); Red=isotype control, Blue= recombinant Cytochrome C antibody.

IHC staining of FFPE human liver with recombinant Cytochrome C antibody (clone CYCS/3128R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human heart lysate with recombinant Cytochrome C antibody. Predicted molecular weight: 12-14 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Cytochrome C antibody (clone CYCS/3128R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P99999