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Cytochrome C (Mitochondrial Marker) Antibody

SKU: orb2320238

Description

Cytochrome C (Mitochondrial Marker) Antibody

Research Area

,Apoptosis|Autophagy|Cardiovascular|Complement System|Lung Cancer|Mitochondria Marker|Transcription Factors

Images & Validation

Tested ApplicationsFC, IHC, WB
Dilution RangeWestern Blot (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityAves, Canine, Drosophila, Equine, Frog, Human, Mouse, Rat
Application Notes
Positive Control: HeLa, Jurkat or PC-3 cells. Human liver or cardiac muscle tissue. Human heart tissue lysate.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone No.CTC05
ImmunogenRecombinant cytochrome c protein
TargetCYCS
Source/Expression SystemBioreactor
Molecular Weight15kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab with BSA and Azide at 200ug/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CYC; CYCS; HCS; THC4

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol

Available Sizes

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20 μg
$ 340.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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