Cytokeratin 20 Antibody / CK20

SKU: orb640077

Description

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 0.1-0.2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG, kappa
• Clone No.: KRT20/3145
• Immunogen: A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the Cytokeratin 20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Cytokeratin 20 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with Cytokeratin 20 antibody (clone KRT20/3145). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone KRT20/3145). Predicted molecular weight ~46 kDa.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 20 antibody (clone KRT20/3145) as confirmation of integrity and purity.

Quick Database Links

UniProt

P35900