Cytokeratin 8, CK8

SKU: orb1294306

Description

Rabbit polyclonal antibody to Cytokeratin 8

Research Area

,Product Categories/Antibodies,Cancer Biomarker

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:2000–1:3000 Immunofluorescence 1:500–1:800 Immunohistochemistry (Paraffin) 1:400–1:600
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Target: Cytokeratin 8, CK8
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-Cytokeratin 8 antibody - at 1/2000 dilution, Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294306 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with orb1294306 at 1/400.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with orb1294306 at 1/400.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294306 Anti-Cytokeratin 8 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Quick Database Links

Gene Symbol

Cytokeratin 8, CK8