DAPI Staining Kit

SKU: orb219885

Description

DAPI (4',6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits -20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

Research Area

Product Categories/Products/ELISA Kits/Food ELISA Kits,Product Categories/Products/ELISA Kits,Product Categories/Tools

Images & Validation

• Application Notes:
  DAPI (4,6-diamidino-2-phenylindole) is a cell permeable fluorescent minor groove-binding probe for DNA. It binds to the double-stranded DNA (especially to AT rich DNA), and forming a stable fluoresces complex. DAPI throughout the live and dead cell membrane that can be utilized for DAN detect for chromosome DAN, Yeast DNA, chloroplast DNA, Virus DNA and so on. DAPI-DNA complex shows light blue flourescence color with excitation light 364 nm and emission light 454 nm.Experiment Notes: This product is 1 mg/ml Chromogen. Solute it with suitable density for applies. Recommend density for tissue staining is 1-2 ug/ml (on the 0.5 ug/ml, flourescence become light saturation). For cell culture the density is 0.1 ug/ml.

  Read more...

Storage & Handling

• Storage: Store at -20°C for one year.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Huanli Zhao 1, Xuejun Wu 2, Shumeng Yang 3, Lili Jiang 4, Huiying Yu 3, Yubin L Formononetin Alleviates the Inflammatory Response Induced by Carotid Balloon Injury in Rats via the PP2A/MAPK Axis Immunol Invest, (2025)

PubMed: 39985449

Applications

IF

Reactivity

Rat

M El Assar, B García-Gómez, JM La Fuente, M Alonso-Isa, JI Martínez-Salamanca Targeting TRPC-5 Channel Inhibition to Improve Penile Vascular Function in Erectile Dysfunction International Journal of Molecular Sciences, (2025)

PubMed:

Zhang, Min et al. Neuroprotective effects of miR-30c on rats with cerebral ischemia/reperfusion injury by targeting SOX9 Pathol Res Pract, 216, 153271 (2020)

PubMed: 33161310