DBC1 Antibody / Deleted in breast cancer 1 / CCAR2

SKU: orb1822896

Description

DBC-1 (deleted in breast cancer gene 1 protein), also known as p30 DBC protein and Cell cycle and apoptosis regulator protein 2 (CCAR2), is one of the genes located within the region of chromosome 8p21.3 that is homozygously deleted in some breast cancers. DBC-1 contains a nuclear localization signal, an N-terminal leucine zipper, an EF hand and a C-terminal coiled-coil region. DBC-1 is closely related to DIS but lacks the SAP domain. During death signaling mediated by TNF-Alpha, endogenous DBC-1 undergoes caspase-dependent processing to generate DBC-1 p120 and p66, both of which include the C-terminus of the protein. Both DBC-1 p120 and p66 relocate to the cytoplasm. Overexpression of the DBC-1 p120 form results in mitochondrial clustering and matrix condensation and increases the sensitivity of cells to TNF-Alpha-mediated apoptosis. In addition, DBC-1 directly interacts with unliganded ER-Alpha, stabilizing its expression and therefore collaborating to suppress apoptosis and promote hormone-independent cell growth.

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: PCRP-KIAA1967-1D10
• Immunogen: Recombinant full-length human CCAR2 / KIAA1967 protein was used as the immunogen for the DBC1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the DBC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This DBC1 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q8N163