Desmoglein-3 (Squamous Cell Marker) Antibody

SKU: orb2318057

Description

Desmoglein-3 (Squamous Cell Marker) Antibody

Research Area

,Apoptosis|Autophagy|Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: A431 cells. Lung squamous cell carcinoma

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5G11
• Immunogen: Recombinant human DSG3 extracellular protein fragment
• Target: DSG3
• Source/Expression System: HEK293 cells
• Molecular Weight: 130kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Quick Database Links

Gene Symbol

DSG3

UniProt

P32926

NCBI Gene ID

1830