DiMethyl-Histone H3-K4 pAb

SKU: orb1258192

This product can be custom made

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Description

DiMethyl-Histone H3-K4 pAb

Research Area

,Signal Transduction

Images & Validation

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Item 1 of 8

Tested Applications	ChIP, IF, IHC, IP, WB
Reactivity	Human, Mouse, Other, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Target	H3K4me2
Molecular Weight	Observed: 18kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H3F3A, H3t, H3.4, H3/g, H3FT

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (orb1258192). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody (orb1258192).

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody (orb1258192) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.