DOG1 Antibody / TMEM16A / ANO1

SKU: orb2640117

Description

DOG 1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: DG1/1484
• Immunogen: A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This DOG 1 antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using DOG 1 antibody (clone DG1/1484). These results demonstrate the foremost specificity of the DG1/1484 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free DOG 1 antibody (clone DG1/1484) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q5XXA6

NCBI Gene ID

55107