DRAM Rabbit Polyclonal Antibody

SKU: orb183254

Description

DRAM Rabbit Polyclonal Antibody

Research Area

Autophagy, Cell Cycle, Metabolism, Signal Transduction, p53 Signaling Pathway

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DRAM (101-200/238aa)
• Target: DRAM1
• Molecular Weight: 26 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Damage regulated autophagy modulator; Damage-regulated autophagy modulator; DNA damage-regulated autophagy modulator protein 1; dram1; DRAM1_HUMAN.

Tissue/Cell: human skin squamous carcinoma, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DRAM Polyclonal Antibody, Unconjugated (orb183254) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DRAM Polyclonal Antibody, Unconjugated (orb183254) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DRAM1

UniProt

Q8N682