DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG M2 Antibody): HRP

SKU: orb413742

Description

Rabbit polyclonal antibody against DYKDDDDK Tag conjugated to HRP

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Tags and Cell Markers | Epitope Tags

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: DYKDDDDK Tag
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DDDDK epitope tag antibody, DDDK antibody, ddk antibody, DYKDDDDK antibody, DYKDDDDK epitope tag antibody, DYKDDDDK tag antibody, ECS epitope tag antibody, ECS tag antibody, Enterokinase Cleavage Site epitope tag antibody, Enterokinase Cleavage Site tag antibody, FLAG antibody, FLAG tag antibody

Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.

Quick Database Links

Gene Symbol

DYKDDDDK Tag