Dynamin (phospho Ser774) Antibody

SKU: orb1273530

Description

Dynamin (phospho Ser774) Antibody

Research Area

,Neuroscience,Phospho-Specific

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Dynamin (Ser774) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
• Target: Dnm1
• Molecular Weight: 95
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Dnm1

UniProt

P21575

RefSeq:P21575