E-Cadherin Antibody [CDH1/1525]

SKU: orb1252364

Description

Research Area

,Cancer,Cell Cycle,Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	FC, IF, IHC-P, WB
Reactivity	Human
Application Notes	Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone No.	CDH1/1525
Immunogen	A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Target	CDH1
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration	0.2 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO

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Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).

Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).

Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).