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Description
Research Area
,Product Categories/Antibodies,EMT
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human E-cadherin |
| Target | E-cadherin (Extracellular) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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E-Cadherin Antibody / CDH1 [orb750019]
FACS, IF, IHC-P, WB
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
20 μg - Item 1 of 5
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Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294382 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Liver carcinoma tissue labeling E-cadherin with orb1294382 at 1/100.
All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.
All lanes: Anti-E cadherin antibody at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294382 overnight at 4°C.
Immunofluorescence of paraffin-embedded sections. Cell: mouse colon; Green: E-cadherin (orb1294382): 1-200; Anti-rabbit 488: 1-300.
Quick Database Links
Gene Symbol
E-cadherin (Extracellular)
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
E-cadherin (Extracellular) (orb1294382)
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