E2F1 Antibody (H357)

SKU: orb38183

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics,Infectious Diseases,Primary Antibodies;Cancer

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7913
• Immunogen: Synthetic Peptide
• Target: E2F1
• Molecular Weight: 46920
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Transcription factor E2F1 antibody, anti-E2F-1 antibody, anti-Retinoblastoma binding protein 3 antibody, anti-RBBP-3 antibody, anti-PRB-binding protein E2F-1 antibody, anti-PBR3 antibody, anti-Retinoblastoma-associated protein 1 antibody, anti-RBAP-1 antibody, anti-E2F1 antibody, anti-RBBP3 antibody

Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in 293T cell line lysate. E2F1 Antibody (H357) (arrow) was detected using the purified Pab.

Anti-E2F1 Antibody (H357) at 1:2000 dilution + Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46. 9 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-E2F1 Antibody (H357) at 1:1000 dilution. Lane 1: NIH-3T3 whole cell lysates. Lane 2: A431 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46. 9 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

E2F1

UniProt

Q01094

RefSeq (Protein):NP_005216.1