EFEMP1 Antibody (N-term)

SKU: orb39786

Description

Rabbit polyclonal antibody to EFEMP1

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Neuroscience;Signal Transduction;Anti-Kinome Antibodies;SARS-Human interaction partners

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22140
• Immunogen: Synthetic Peptide
• Target: EFEMP1
• Molecular Weight: 54641
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Extracellular protein S1-5 antibody, anti-Fibrillin-like protein antibody, anti-Fibulin-3 antibody, anti-FIBL-3 antibody, anti-EGF-containing fibulin-like extracellular matrix protein 1 antibody, anti-FBNL antibody, anti-FBLN3 antibody, anti-EFEMP1 antibody

Western blot analysis of EFEMP1 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). EFEMP1 (arrow) was detected using the purified Pab.

EFEMP1 Antibody (N-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EFEMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-EFEMP1 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EFEMP1

UniProt

Q12805

RefSeq (Protein):NP_001034437.1, NP_001034438.1