EHHADH Antibody (C-term)

SKU: orb39323

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Metabolism;Signal Transduction;Catalog Updated

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22585
• Immunogen: Synthetic Peptide
• Target: EHHADH
• Molecular Weight: 79495
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Peroxisomal bifunctional enzyme antibody, anti-PBE antibody, anti-PBFE antibody, anti-Enoyl-CoA hydratase/3, 2-trans-enoyl-CoA isomerase antibody, anti-3-hydroxyacyl-CoA dehydrogenase antibody, anti-ECHD antibody, anti-EHHADH antibody

Western blot analysis of EHHADH Antibody (C-term) in mouse liver (lane 1), kidney (lane 2) tissue lysates (35 ug/lane). EHHADH (arrow) was detected using the purified Pab.

Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

EHHADH

UniProt

Q08426

RefSeq (Protein):NP_001159887.1, NP_001957.2