eIF4B (phospho Ser422) Antibody

SKU: orb1253698

Description

eIF4B (phospho Ser422) Antibody

Research Area

,Phospho-Specific

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: eIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B.
• Target: EIF4B
• Molecular Weight: 80 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIF-4B, PRO1843, ESR, NR3A1,

Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using eIF4B (phospho-Ser422).

Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B (Phospho-Ser422).

Quick Database Links

Gene Symbol

EIF4B

UniProt

P23588

RefSeq (Protein):NP_001408.2