ENaC beta Antibody: FITC

SKU: orb152735

Description

Rabbit polyclonal to ENaC Beta (FITC). The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport. Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption. ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta , and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate. The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Epigenetics,Neuroscience | Neurotransmission | Ion Channels | Sodium Channels | Epithelial Sodium Channels (ENaC)

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), IHC (1:100)
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Target: ENaC
• Molecular Weight: 87kDa
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SCNN1B antibody, Amiloride sensitive sodium channel subunit beta antibody, Amiloride-sensitive sodium channel beta-subunit antibody, Beta ENaC antibody, Beta NaCH antibody, ENaC beta antibody, ENaCB antibody, Epithelial Na(+) channel subunit beta antibody, Epithelial Na+ channel beta subunit antibody, Epithelial Na+ channel subunit beta antibody, Epithelial sodium channel beta 2 subunit antibody, Epithelial sodium channel beta 3 subunit antibody, Nonvoltage gated sodium channel 1 beta subunit antibody, Nonvoltage gated sodium channel 1 subunit beta antibody, Nonvoltage-gated sodium channel 1 beta subunit antibody, SCNEB antibody, SCNN 1B antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody, Sodium channel nonvoltage gated 1 beta antibody

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse mpkCCD cell lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-8).

Quick Database Links

Gene Symbol

ENaC

UniProt

P37090

NCBI Gene ID

24767

RefSeq (Protein):NP_036780