ENO1 Antibody (Center)

SKU: orb36654

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Epigenetics,Crown Antibodies;Primary Antibodies;Cancer;Metabolism;Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Other, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19620
• Immunogen: Synthetic Peptide
• Target: ENO1
• Molecular Weight: 47169
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Alpha-enolase antibody, anti-2-phospho-D-glycerate hydro-lyase antibody, anti-Non-neural enolase antibody, anti-NNE antibody, anti-Enolase 1 antibody, anti-Phosphopyruvate hydratase antibody, anti-C-myc promoter-binding protein antibody, anti-MBP-1 antibody, anti-MPB-1 antibody, anti-Plasminogen-binding protein antibody, anti-MPB1 antibody, anti-MBPB1 antibody, anti-ENO1L1 antibody, anti-ENO1 antibody

ENO1 Antibody (Center) western blot analysis in Jurkat, MCF-7, U251, mouse NIH/3T3 cell line and mouse brain tissue lysates (35 ug/lane). This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).

ENO1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody (Center). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.

Quick Database Links

Gene Symbol

ENO1

UniProt

P06733

RefSeq (Protein):NP_001188412.1, NP_001419.1