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ENO1 Antibody
SKU: orb1788430
Description
Research Area
,Primary Antibodies
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b,k |
| Immunogen | 1-415 aa |
| Target | Purified His-tagged ENO1 protein was used to produced this monoclonal antibody. |
| Molecular Weight | 47169 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ENO1;ENO1L1; MBPB1; MPB1; Alpha-enolase; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; Alpha-enolase; C-myc promoter-binding protein; Alpha-enolase; Enolase 1; Alpha-enolase; MBP-1; Alpha-enolase; MPB-1; Alpha-enolase; Non-neural enolase; Alpha-enolase; Phosphopyruvate hydratase; Alpha-enolase; Plasminogen-binding protein
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Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from MCF-7 cell line, mouse stomach, mouse liver tissue lysate, Hela, HepG2, NIH/3T3, A431 cell line (from left to right), using ENO1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Quick Database Links
Gene Symbol
Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
UniProt
RefSeq (Protein):NP_001188412.1, NP_001419.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001188412.1, NP_001419.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ENO1 Antibody (orb1788430)
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