ENPP2 Antibody (Center)

SKU: orb1931393

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies;Cancer;Cardiovascular;Metabolism;Neuroscience;Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, WB: 1:2000, WB: 1:2000, FC: 1:10~50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB18117
• Target: This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.
• Molecular Weight: 98994 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2

ENPP2 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of (arrow) using rabbit polyclonal ENPP2 Antibody (Center). 293T cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the gene.

Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 99 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 99 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from human placenta and plasma tissue lysate (from left to right), using ENPP2 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.

UniProt

Q13822

RefSeq (Protein):NP_001035181.1, NP_006200.3, NP_001124335.1