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Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2. |
| Target | ENTPD2 |
| Molecular Weight | 54 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−ENTPD2 purified MaxPab mouse polyclonal antibody (B01P) [orb2294803]
WB
Human
Mouse
Polyclonal
Unconjugated
50 μgENTPD2 Rabbit Polyclonal Antibody (HRP) [orb482498]
WB
Bovine, Gallus, Human, Mouse, Sheep
Rat
Rabbit
Polyclonal
HRP
100 μl

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Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of ENTPD2 Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
ENTPD2 Antibody (orb1262264)
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