Eotaxin (CCL11) Antibody (biotin)

SKU: orb1671454

Description

Eotaxin (CCL11) Antibody (biotin)

Research Area

,Chemokines & Cytokines,Immunology,Other

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Application Notes:
  Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin. Western Blot To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography and then biotinylated.
• Target: Ccl11
• Purification: Eotaxin specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Unconjugated

Storage & Handling

• Storage: Antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CCL11

To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin.

To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

Ccl11

UniProt

P48298

RefSeq:P48298