EpCAM Antibody / Extracellular domain

SKU: orb2640530

Description

EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot:1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Canine, Feline, Human
• Application Notes:
  Titering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: EGP40/2041R
• Immunogen: A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant EpCAM antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human MCF7 cell lysate with recombinant EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Western blot testing of human lung lysate with EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.

IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.

Quick Database Links

UniProt

P16422