EpCAM Antibody

SKU: orb2640322

Description

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Images & Validation

• Tested Applications: ELISA, FACS, IF, IHC-P
• Dilution Range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Canine, Feline, Human
• Application Notes:
  Titering of the Ep-CAM antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: EGP40/1798
• Immunogen: A human recombinant partial protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Ep-CAM antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P16422